Mackenzie-Graham, A, Lee, E, Velan, S, Ruffins, S, Jacobs, R, Dinov, I, Lee, A, McClay, W, Capetillo, L, Crawford, K, Burns, G, Toga, A. A multimodal, multidimensional atlas of the C57BL/6 Mouse Brain, Neurosci. Letter, Vol. 27, CD ROM, abs. 2001,

---

Abstract
Molecular geneticists produce genetically modified animals at an ever-increasing rate. A comprehensive framework to encompass genetic information in the context of imaging of anatomic and architectural data holds tremendous promise for producing new insights into the relationship between genotype and phenotype. We have, and continue to develop, an innovative multimodality, multidimensional atlas of the C57BL/6J mouse brain. Magnetic resonance imaging has revolutionized the non-invasive investigation of neuroanatomy and function, and is an integral component of our in vivo digital atlas. Blockface imaging allows the visualization of the brain free of many of the artifacts that affect serially-stained sections. Nissl- and myelin-stained sections provide a wealth of information about the topography of cortical and subcortical nuclei. These data were reconstructed into 3D volumes, quantitatively transformed into a defined coordinate system, delineated and described anatomically. Our Mouse Atlas Visualization package is a self-contained, platform independent software tool that can visualize these data sets simultaneously. The goal of the Mouse Atlas Project is to confer the ability to compare new maps of gene expression with a conveniently accessible inventory of digital brain maps that will enable the geneticist to leverage the results of brain mapping efforts in their investigations of gene function. Supported by: MH61223

---

\Ivo D. Dinov, Ph.D., Lab of Neuro Imaging, UCLA School of Medicine/